D6171

Sigma

 

Dulbecco’s Modified Eagle’s Medium - high glucose

HEPES modification, With 4500 mg/L glucose, 25 mM HEPES, and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, cell culture tested

Synonym:DME, DMEM

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Description

ApplicationDulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
ReconstitutionSupplement with 0.584 g/L L-glutamine.

Properties

sterilitysterile-filtered
formliquid
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.2-8°C